Construct: shRNA TRCN0000305782
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172300.3-1444s21c1
- DNA Barcode:
- CTCTGCAGGGCCAGCTAATTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ube2z (268470)
- Gene Description:
- ubiquitin-conjugating enzyme E2Z
- Transcript:
- RefSeq NM_172300.3 (CURRENT)
- Match location:
- Position 1444 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000305782)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTCTGCAGGGCCAGCTAATTA
- Hairpin Sequence:
-
5'-CCGG-CTCTGCAGGGCCAGCTAATTA-CTCGAG-TAATTAGCTGGCCCTGCAGAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTCTGCAGGGCCAGCTAATTACTCGAGTAATTAGCTGGCCCTGCAGAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTCTGCAGGGCCAGCTAATTACTCGAGTAATTAGCTGGCCCTGCAGAG-3'
Other clones with same target sequence:
(none)
