Construct: shRNA TRCN0000305999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031170.2-845s21c1
DNA Barcode:
ACGTCTGTGGTGCTGTCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krt8 (16691)
Gene Description:
keratin 8
Transcript:
RefSeq NM_031170.2 (CURRENT)
Match location:
Position 845 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCTGTGGTGCTGTCTATG
Hairpin Sequence:
5'-CCGG-ACGTCTGTGGTGCTGTCTATG-CTCGAG-CATAGACAGCACCACAGACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCTGTGGTGCTGTCTATGCTCGAGCATAGACAGCACCACAGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCTGTGGTGCTGTCTATGCTCGAGCATAGACAGCACCACAGACGT-3'

Other clones with same target sequence:

(none)