Construct: shRNA TRCN0000306648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016677.4-1299s21c1
DNA Barcode:
TACGGTGAGATGGACTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hpcal1 (53602)
Gene Description:
hippocalcin-like 1
Transcript:
RefSeq NM_016677.4 (CURRENT)
Match location:
Position 1299 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000306648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGGTGAGATGGACTATAAT
Hairpin Sequence:
5'-CCGG-TACGGTGAGATGGACTATAAT-CTCGAG-ATTATAGTCCATCTCACCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGGTGAGATGGACTATAATCTCGAGATTATAGTCCATCTCACCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGGTGAGATGGACTATAATCTCGAGATTATAGTCCATCTCACCGTA-3'

Other clones with same target sequence:

(none)