Construct: shRNA TRCN0000307410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022023.2-868s21c1
DNA Barcode:
CTATAGGAAGGTGGGTATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gmfb (63985)
Gene Description:
glia maturation factor, beta
Transcript:
RefSeq NM_022023.2 (CURRENT)
Match location:
Position 868 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATAGGAAGGTGGGTATTAG
Hairpin Sequence:
5'-CCGG-CTATAGGAAGGTGGGTATTAG-CTCGAG-CTAATACCCACCTTCCTATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATAGGAAGGTGGGTATTAGCTCGAGCTAATACCCACCTTCCTATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATAGGAAGGTGGGTATTAGCTCGAGCTAATACCCACCTTCCTATAG-3'

Other clones with same target sequence:

(none)