Construct: shRNA TRCN0000308600

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009626.4-393s21c1
DNA Barcode:
CCCTCTTTCTACCACAGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adh7 (11529)
Gene Description:
alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
Transcript:
RefSeq NM_009626.4 (CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308600)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTTTCTACCACAGTGTA
Hairpin Sequence:
5'-CCGG-CCCTCTTTCTACCACAGTGTA-CTCGAG-TACACTGTGGTAGAAAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTTTCTACCACAGTGTACTCGAGTACACTGTGGTAGAAAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTTTCTACCACAGTGTACTCGAGTACACTGTGGTAGAAAGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000042018