Construct: shRNA TRCN0000308621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174874.3-130s21c1
DNA Barcode:
GACGAAATCTTGTCTGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg4b (66615)
Gene Description:
autophagy related 4B, cysteine peptidase
Transcript:
RefSeq NM_174874.3 (CURRENT)
Match location:
Position 130 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGAAATCTTGTCTGATGTT
Hairpin Sequence:
5'-CCGG-GACGAAATCTTGTCTGATGTT-CTCGAG-AACATCAGACAAGATTTCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGAAATCTTGTCTGATGTTCTCGAGAACATCAGACAAGATTTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGAAATCTTGTCTGATGTTCTCGAGAACATCAGACAAGATTTCGTC-3'

Other clones with same target sequence:

  1. TRCN0000030942