Construct: shRNA TRCN0000308803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023294.2-1246s21c1
DNA Barcode:
GCTGACATTGAGAGAATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndc80 (67052)
Gene Description:
NDC80 kinetochore complex component
Transcript:
RefSeq NM_023294.2 (CURRENT)
Match location:
Position 1246 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACATTGAGAGAATAAAT
Hairpin Sequence:
5'-CCGG-GCTGACATTGAGAGAATAAAT-CTCGAG-ATTTATTCTCTCAATGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACATTGAGAGAATAAATCTCGAGATTTATTCTCTCAATGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACATTGAGAGAATAAATCTCGAGATTTATTCTCTCAATGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000088288