Construct: shRNA TRCN0000308921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028151.2-3155s21c1
DNA Barcode:
GTTATCCACCACCTGTCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Skiv2l2 (72198)
Gene Description:
superkiller viralicidic activity 2-like 2 (S. cerevisiae)
Transcript:
RefSeq NM_028151.2 (CURRENT)
Match location:
Position 3155 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTATCCACCACCTGTCTGAT
Hairpin Sequence:
5'-CCGG-GTTATCCACCACCTGTCTGAT-CTCGAG-ATCAGACAGGTGGTGGATAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTATCCACCACCTGTCTGATCTCGAGATCAGACAGGTGGTGGATAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTATCCACCACCTGTCTGATCTCGAGATCAGACAGGTGGTGGATAAC-3'

Other clones with same target sequence:

  1. TRCN0000112070