Construct: shRNA TRCN0000309449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003908.1-3357s21c1
DNA Barcode:
GCTCCAGATATTGCCAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cltc (67300)
Gene Description:
clathrin, heavy polypeptide (Hc)
Transcript:
RefSeq NM_001003908.1 (CURRENT)
Match location:
Position 3357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCAGATATTGCCAACATT
Hairpin Sequence:
5'-CCGG-GCTCCAGATATTGCCAACATT-CTCGAG-AATGTTGGCAATATCTGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCAGATATTGCCAACATTCTCGAGAATGTTGGCAATATCTGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCAGATATTGCCAACATTCTCGAGAATGTTGGCAATATCTGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000113162