Construct: shRNA TRCN0000309576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172697.3-1167s21c1
DNA Barcode:
GCATCTTGACTTTGGTCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prpf38a (230596)
Gene Description:
PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
Transcript:
RefSeq NM_172697.3 (CURRENT)
Match location:
Position 1167 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTTGACTTTGGTCCAAA
Hairpin Sequence:
5'-CCGG-GCATCTTGACTTTGGTCCAAA-CTCGAG-TTTGGACCAAAGTCAAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTTGACTTTGGTCCAAACTCGAGTTTGGACCAAAGTCAAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTTGACTTTGGTCCAAACTCGAGTTTGGACCAAAGTCAAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000123759