Construct: shRNA TRCN0000309697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144553.2-800s21c1
DNA Barcode:
GCAGGAATGTTCCTAAAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlgap5 (218977)
Gene Description:
discs, large (Drosophila) homolog-associated protein 5
Transcript:
RefSeq NM_144553.2 (CURRENT)
Match location:
Position 800 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAATGTTCCTAAAGCAA
Hairpin Sequence:
5'-CCGG-GCAGGAATGTTCCTAAAGCAA-CTCGAG-TTGCTTTAGGAACATTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAATGTTCCTAAAGCAACTCGAGTTGCTTTAGGAACATTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAATGTTCCTAAAGCAACTCGAGTTGCTTTAGGAACATTCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000089135