Construct: shRNA TRCN0000312114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011631.1-1355s21c1
DNA Barcode:
CCATGATATGATGCCCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsp90b1 (22027)
Gene Description:
heat shock protein 90, beta (Grp94), member 1
Transcript:
RefSeq NM_011631.1 (CURRENT)
Match location:
Position 1355 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGATATGATGCCCAAATA
Hairpin Sequence:
5'-CCGG-CCATGATATGATGCCCAAATA-CTCGAG-TATTTGGGCATCATATCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGATATGATGCCCAAATACTCGAGTATTTGGGCATCATATCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGATATGATGCCCAAATACTCGAGTATTTGGGCATCATATCATGG-3'

Other clones with same target sequence:

  1. TRCN0000071924