Construct: shRNA TRCN0000312184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009949.2-1172s21c1
DNA Barcode:
GCTGGTTTGATAAGTCCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpt2 (12896)
Gene Description:
carnitine palmitoyltransferase 2
Transcript:
RefSeq NM_009949.2 (CURRENT)
Match location:
Position 1172 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTTGATAAGTCCTTTA
Hairpin Sequence:
5'-CCGG-GCTGGTTTGATAAGTCCTTTA-CTCGAG-TAAAGGACTTATCAAACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTTGATAAGTCCTTTACTCGAGTAAAGGACTTATCAAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTTGATAAGTCCTTTACTCGAGTAAAGGACTTATCAAACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000110501