Construct: shRNA TRCN0000312188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019998.3-675s21c1
DNA Barcode:
GCTCTACCCATCTCTGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alg2 (56737)
Gene Description:
asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase)
Transcript:
RefSeq NM_019998.3 (CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTACCCATCTCTGAATAT
Hairpin Sequence:
5'-CCGG-GCTCTACCCATCTCTGAATAT-CTCGAG-ATATTCAGAGATGGGTAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTACCCATCTCTGAATATCTCGAGATATTCAGAGATGGGTAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTACCCATCTCTGAATATCTCGAGATATTCAGAGATGGGTAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000110467