Construct: shRNA TRCN0000312884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144825.2-3859s21c1
DNA Barcode:
TAATTGGGATGTCATAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taok1 (216965)
Gene Description:
TAO kinase 1
Transcript:
RefSeq NM_144825.2 (CURRENT)
Match location:
Position 3859 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATTGGGATGTCATAGTATT
Hairpin Sequence:
5'-CCGG-TAATTGGGATGTCATAGTATT-CTCGAG-AATACTATGACATCCCAATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATTGGGATGTCATAGTATTCTCGAGAATACTATGACATCCCAATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATTGGGATGTCATAGTATTCTCGAGAATACTATGACATCCCAATTA-3'

Other clones with same target sequence:

(none)