Construct: shRNA TRCN0000313108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011766.5-3459s21c1
DNA Barcode:
GGATAAAGCCAAGCGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfpm2 (22762)
Gene Description:
zinc finger protein, multitype 2
Transcript:
RefSeq NM_011766.5 (CURRENT)
Match location:
Position 3459 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATAAAGCCAAGCGATTATA
Hairpin Sequence:
5'-CCGG-GGATAAAGCCAAGCGATTATA-CTCGAG-TATAATCGCTTGGCTTTATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATAAAGCCAAGCGATTATACTCGAGTATAATCGCTTGGCTTTATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATAAAGCCAAGCGATTATACTCGAGTATAATCGCTTGGCTTTATCC-3'

Other clones with same target sequence:

(none)