Construct: shRNA TRCN0000313597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008942.2-3187s21c1
DNA Barcode:
CTCCTGTTAGTGACGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Npepps (19155)
Gene Description:
aminopeptidase puromycin sensitive
Transcript:
RefSeq NM_008942.2 (CURRENT)
Match location:
Position 3187 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTGTTAGTGACGGATATT
Hairpin Sequence:
5'-CCGG-CTCCTGTTAGTGACGGATATT-CTCGAG-AATATCCGTCACTAACAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTGTTAGTGACGGATATTCTCGAGAATATCCGTCACTAACAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTGTTAGTGACGGATATTCTCGAGAATATCCGTCACTAACAGGAG-3'

Other clones with same target sequence:

(none)