Construct: shRNA TRCN0000313736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016719.1-429s21c1
DNA Barcode:
AGGAAGAAGCAGGTGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grb14 (50915)
Gene Description:
growth factor receptor bound protein 14
Transcript:
RefSeq NM_016719.1 (CURRENT)
Match location:
Position 429 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAAGAAGCAGGTGATTAAA
Hairpin Sequence:
5'-CCGG-AGGAAGAAGCAGGTGATTAAA-CTCGAG-TTTAATCACCTGCTTCTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAAGAAGCAGGTGATTAAACTCGAGTTTAATCACCTGCTTCTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAAGAAGCAGGTGATTAAACTCGAGTTTAATCACCTGCTTCTTCCT-3'

Other clones with same target sequence:

(none)