Construct: shRNA TRCN0000313818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022316.2-1252s21c1
DNA Barcode:
GACATGGTTCAGGCCATTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smoc1 (64075)
Gene Description:
SPARC related modular calcium binding 1
Transcript:
RefSeq NM_022316.2 (CURRENT)
Match location:
Position 1252 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATGGTTCAGGCCATTAAC
Hairpin Sequence:
5'-CCGG-GACATGGTTCAGGCCATTAAC-CTCGAG-GTTAATGGCCTGAACCATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATGGTTCAGGCCATTAACCTCGAGGTTAATGGCCTGAACCATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATGGTTCAGGCCATTAACCTCGAGGTTAATGGCCTGAACCATGTC-3'

Other clones with same target sequence:

  1. TRCN0000373667