Construct: shRNA TRCN0000314335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139309.4-2196s21c1
DNA Barcode:
GCAGTGGTACTTGCTGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fktn (246179)
Gene Description:
fukutin
Transcript:
RefSeq NM_139309.4 (CURRENT)
Match location:
Position 2196 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGGTACTTGCTGATAAA
Hairpin Sequence:
5'-CCGG-GCAGTGGTACTTGCTGATAAA-CTCGAG-TTTATCAGCAAGTACCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGGTACTTGCTGATAAACTCGAGTTTATCAGCAAGTACCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGGTACTTGCTGATAAACTCGAGTTTATCAGCAAGTACCACTGC-3'

Other clones with same target sequence:

  1. TRCN0000194045