Construct: shRNA TRCN0000314444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011339.2-595s21c1
DNA Barcode:
CGTTCTCATTCTTATACTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cxcl15 (20309)
Gene Description:
chemokine (C-X-C motif) ligand 15
Transcript:
RefSeq NM_011339.2 (CURRENT)
Match location:
Position 595 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCTCATTCTTATACTCTA
Hairpin Sequence:
5'-CCGG-CGTTCTCATTCTTATACTCTA-CTCGAG-TAGAGTATAAGAATGAGAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCTCATTCTTATACTCTACTCGAGTAGAGTATAAGAATGAGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCTCATTCTTATACTCTACTCGAGTAGAGTATAAGAATGAGAACG-3'

Other clones with same target sequence:

  1. TRCN0000112210