Construct: shRNA TRCN0000315504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007533.5-1176s21c1
DNA Barcode:
GCAGTCACGAAAGAAGGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bckdha (12039)
Gene Description:
branched chain ketoacid dehydrogenase E1, alpha polypeptide
Transcript:
RefSeq NM_007533.5 (CURRENT)
Match location:
Position 1176 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCACGAAAGAAGGTCAT
Hairpin Sequence:
5'-CCGG-GCAGTCACGAAAGAAGGTCAT-CTCGAG-ATGACCTTCTTTCGTGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCACGAAAGAAGGTCATCTCGAGATGACCTTCTTTCGTGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCACGAAAGAAGGTCATCTCGAGATGACCTTCTTTCGTGACTGC-3'

Other clones with same target sequence:

  1. TRCN0000041384