Construct: shRNA TRCN0000316118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019788.3-1881s21c1
DNA Barcode:
GCTTAAAGAAAGGCATAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bloc1s6 (18457)
Gene Description:
biogenesis of lysosomal organelles complex-1, subunit 6, pallidin
Transcript:
RefSeq NM_019788.3 (CURRENT)
Match location:
Position 1881 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAAAGAAAGGCATAACTT
Hairpin Sequence:
5'-CCGG-GCTTAAAGAAAGGCATAACTT-CTCGAG-AAGTTATGCCTTTCTTTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAAAGAAAGGCATAACTTCTCGAGAAGTTATGCCTTTCTTTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAAAGAAAGGCATAACTTCTCGAGAAGTTATGCCTTTCTTTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000125574