Construct: shRNA TRCN0000316124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025433.3-287s21c1
DNA Barcode:
CCTTCGTTATACGCATGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl7l1 (66229)
Gene Description:
ribosomal protein L7-like 1
Transcript:
RefSeq NM_025433.3 (CURRENT)
Match location:
Position 287 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGTTATACGCATGGAAA
Hairpin Sequence:
5'-CCGG-CCTTCGTTATACGCATGGAAA-CTCGAG-TTTCCATGCGTATAACGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGTTATACGCATGGAAACTCGAGTTTCCATGCGTATAACGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGTTATACGCATGGAAACTCGAGTTTCCATGCGTATAACGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000096775