Transcript: Mouse NM_025433.3

Mus musculus ribosomal protein L7-like 1 (Rpl7l1), mRNA.

Source:
NCBI, updated 2019-02-23
Taxon:
Mus musculus (mouse)
Gene:
Rpl7l1 (66229)
Length:
2606
CDS:
25..765

Additional Resources:

NCBI RefSeq record:
NM_025433.3
NBCI Gene record:
Rpl7l1 (66229)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025433.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000096777 GATCGGATCAATCAGCTCATT pLKO.1 730 CDS 100% 4.950 3.960 N Rpl7l1 n/a
2 TRCN0000316125 GATCGGATCAATCAGCTCATT pLKO_005 730 CDS 100% 4.950 3.960 N Rpl7l1 n/a
3 TRCN0000096776 GCCAAGCAAAGATTAACAATA pLKO.1 491 CDS 100% 13.200 9.240 N Rpl7l1 n/a
4 TRCN0000316199 GCCAAGCAAAGATTAACAATA pLKO_005 491 CDS 100% 13.200 9.240 N Rpl7l1 n/a
5 TRCN0000096774 GCCTTCTATCTTGGTGTTTAA pLKO.1 1019 3UTR 100% 13.200 9.240 N Rpl7l1 n/a
6 TRCN0000096778 CGACTAGAATCTTTCGTGCAT pLKO.1 175 CDS 100% 2.640 1.848 N Rpl7l1 n/a
7 TRCN0000096775 CCTTCGTTATACGCATGGAAA pLKO.1 287 CDS 100% 4.950 2.970 N Rpl7l1 n/a
8 TRCN0000316124 CCTTCGTTATACGCATGGAAA pLKO_005 287 CDS 100% 4.950 2.970 N Rpl7l1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025433.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05409 pDONR223 100% 84.6% 83.4% None (many diffs) n/a
2 ccsbBroad304_05409 pLX_304 0% 84.6% 83.4% V5 (many diffs) n/a
3 TRCN0000474109 TTTCTCAGGTAGGCTTATACGCCG pLX_317 52.2% 84.6% 83.4% V5 (many diffs) n/a
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