Construct: shRNA TRCN0000316375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007626.3-530s21c1
DNA Barcode:
CGGTGACTTAATGTTCTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbx5 (12419)
Gene Description:
chromobox 5
Transcript:
RefSeq NM_007626.3 (CURRENT)
Match location:
Position 530 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGACTTAATGTTCTTAAT
Hairpin Sequence:
5'-CCGG-CGGTGACTTAATGTTCTTAAT-CTCGAG-ATTAAGAACATTAAGTCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGACTTAATGTTCTTAATCTCGAGATTAAGAACATTAAGTCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGACTTAATGTTCTTAATCTCGAGATTAAGAACATTAAGTCACCG-3'

Other clones with same target sequence:

  1. TRCN0000071051