Construct: shRNA TRCN0000071051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007626.2-465s1c1
DNA Barcode:
CGGTGACTTAATGTTCTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbx5 (12419)
Gene Description:
chromobox 5
Transcript:
RefSeq NM_007626.2 (NON-CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGACTTAATGTTCTTAAT
Hairpin Sequence:
5'-CCGG-CGGTGACTTAATGTTCTTAAT-CTCGAG-ATTAAGAACATTAAGTCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGACTTAATGTTCTTAATCTCGAGATTAAGAACATTAAGTCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGACTTAATGTTCTTAATCTCGAGATTAAGAACATTAAGTCACCG-3'

Other clones with same target sequence:

  1. TRCN0000316375