Construct: shRNA TRCN0000316462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146153.3-2768s21c1
DNA Barcode:
GCCCAAGAGCAAGAAGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thrap3 (230753)
Gene Description:
thyroid hormone receptor associated protein 3
Transcript:
RefSeq NM_146153.3 (CURRENT)
Match location:
Position 2768 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAAGAGCAAGAAGTATTA
Hairpin Sequence:
5'-CCGG-GCCCAAGAGCAAGAAGTATTA-CTCGAG-TAATACTTCTTGCTCTTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAAGAGCAAGAAGTATTACTCGAGTAATACTTCTTGCTCTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAAGAGCAAGAAGTATTACTCGAGTAATACTTCTTGCTCTTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000095128