Construct: shRNA TRCN0000316695

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019990.4-519s21c1
DNA Barcode:
CCTGAAGAACCGTGATGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stard10 (56018)
Gene Description:
START domain containing 10
Transcript:
RefSeq NM_019990.4 (CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316695)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGAACCGTGATGTCAT
Hairpin Sequence:
5'-CCGG-CCTGAAGAACCGTGATGTCAT-CTCGAG-ATGACATCACGGTTCTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGAACCGTGATGTCATCTCGAGATGACATCACGGTTCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGAACCGTGATGTCATCTCGAGATGACATCACGGTTCTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000156337
  2. TRCN0000105062