Construct: shRNA TRCN0000316949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023585.4-413s21c1
DNA Barcode:
CGTCTTATGATGTCCAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2v2 (70620)
Gene Description:
ubiquitin-conjugating enzyme E2 variant 2
Transcript:
RefSeq NM_023585.4 (CURRENT)
Match location:
Position 413 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTTATGATGTCCAAAGAA
Hairpin Sequence:
5'-CCGG-CGTCTTATGATGTCCAAAGAA-CTCGAG-TTCTTTGGACATCATAAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTTATGATGTCCAAAGAACTCGAGTTCTTTGGACATCATAAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTTATGATGTCCAAAGAACTCGAGTTCTTTGGACATCATAAGACG-3'

Other clones with same target sequence:

  1. TRCN0000040914