Construct: shRNA TRCN0000317138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015747.2-891s21c1
DNA Barcode:
CCCATTGTATTGTCGGTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc20a1 (20515)
Gene Description:
solute carrier family 20, member 1
Transcript:
RefSeq NM_015747.2 (CURRENT)
Match location:
Position 891 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTGTATTGTCGGTGCAA
Hairpin Sequence:
5'-CCGG-CCCATTGTATTGTCGGTGCAA-CTCGAG-TTGCACCGACAATACAATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTGTATTGTCGGTGCAACTCGAGTTGCACCGACAATACAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTGTATTGTCGGTGCAACTCGAGTTGCACCGACAATACAATGGG-3'

Other clones with same target sequence:

  1. TRCN0000068404