Construct: shRNA TRCN0000317324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133999.1-1131s21c1
DNA Barcode:
CGCAGGCAGTTACTCTTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fig4 (103199)
Gene Description:
FIG4 phosphoinositide 5-phosphatase
Transcript:
RefSeq NM_133999.1 (CURRENT)
Match location:
Position 1131 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGGCAGTTACTCTTCTTA
Hairpin Sequence:
5'-CCGG-CGCAGGCAGTTACTCTTCTTA-CTCGAG-TAAGAAGAGTAACTGCCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGGCAGTTACTCTTCTTACTCGAGTAAGAAGAGTAACTGCCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGGCAGTTACTCTTCTTACTCGAGTAAGAAGAGTAACTGCCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000124712