Construct: shRNA TRCN0000317524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175641.2-2666s21c1
DNA Barcode:
GATGTACTGATGTGGATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ltbp4 (108075)
Gene Description:
latent transforming growth factor beta binding protein 4
Transcript:
RefSeq NM_175641.2 (CURRENT)
Match location:
Position 2666 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTACTGATGTGGATGAAT
Hairpin Sequence:
5'-CCGG-GATGTACTGATGTGGATGAAT-CTCGAG-ATTCATCCACATCAGTACATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTACTGATGTGGATGAATCTCGAGATTCATCCACATCAGTACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTACTGATGTGGATGAATCTCGAGATTCATCCACATCAGTACATC-3'

Other clones with same target sequence:

  1. TRCN0000065790