Construct: shRNA TRCN0000317688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145148.2-1375s21c1
DNA Barcode:
CCCGAAGGATTTCAGTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frmd4b (232288)
Gene Description:
FERM domain containing 4B
Transcript:
RefSeq NM_145148.2 (CURRENT)
Match location:
Position 1375 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAAGGATTTCAGTGTCTA
Hairpin Sequence:
5'-CCGG-CCCGAAGGATTTCAGTGTCTA-CTCGAG-TAGACACTGAAATCCTTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAAGGATTTCAGTGTCTACTCGAGTAGACACTGAAATCCTTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAAGGATTTCAGTGTCTACTCGAGTAGACACTGAAATCCTTCGGG-3'

Other clones with same target sequence:

  1. TRCN0000054995