Transcript: Mouse NM_145148.2

Mus musculus FERM domain containing 4B (Frmd4b), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Frmd4b (232288)
Length:
5043
CDS:
591..3536

Additional Resources:

NCBI RefSeq record:
NM_145148.2
NBCI Gene record:
Frmd4b (232288)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145148.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000054994 CGGTTACTATATCGCTGGATA pLKO.1 2837 CDS 100% 4.950 3.960 N Frmd4b n/a
2 TRCN0000317689 CGGTTACTATATCGCTGGATA pLKO_005 2837 CDS 100% 4.950 3.960 N Frmd4b n/a
3 TRCN0000054996 CCAGTCAAGCTCCGAGTATTA pLKO.1 2633 CDS 100% 13.200 9.240 N Frmd4b n/a
4 TRCN0000054997 CCAGTATGACTTACAAGACAA pLKO.1 1268 CDS 100% 4.950 3.465 N Frmd4b n/a
5 TRCN0000317687 CCAGTATGACTTACAAGACAA pLKO_005 1268 CDS 100% 4.950 3.465 N Frmd4b n/a
6 TRCN0000054995 CCCGAAGGATTTCAGTGTCTA pLKO.1 1375 CDS 100% 4.950 3.465 N Frmd4b n/a
7 TRCN0000317688 CCCGAAGGATTTCAGTGTCTA pLKO_005 1375 CDS 100% 4.950 3.465 N Frmd4b n/a
8 TRCN0000054993 GCGTCGCATTTCAACCTGAAA pLKO.1 693 CDS 100% 4.950 3.465 N Frmd4b n/a
9 TRCN0000317761 GCGTCGCATTTCAACCTGAAA pLKO_005 693 CDS 100% 4.950 3.465 N Frmd4b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145148.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.