Construct: shRNA TRCN0000317806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177732.4-562s21c1
DNA Barcode:
GCCTTTGATCTGGAAGGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc35d1 (242585)
Gene Description:
solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
Transcript:
RefSeq NM_177732.4 (CURRENT)
Match location:
Position 562 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTGATCTGGAAGGATAT
Hairpin Sequence:
5'-CCGG-GCCTTTGATCTGGAAGGATAT-CTCGAG-ATATCCTTCCAGATCAAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTGATCTGGAAGGATATCTCGAGATATCCTTCCAGATCAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTGATCTGGAAGGATATCTCGAGATATCCTTCCAGATCAAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000079505