Construct: shRNA TRCN0000317997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008973.2-1706s21c1
DNA Barcode:
CCATGAAGACTCAGAGATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptn (19242)
Gene Description:
pleiotrophin
Transcript:
RefSeq NM_008973.2 (CURRENT)
Match location:
Position 1706 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAAGACTCAGAGATGTA
Hairpin Sequence:
5'-CCGG-CCATGAAGACTCAGAGATGTA-CTCGAG-TACATCTCTGAGTCTTCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAAGACTCAGAGATGTACTCGAGTACATCTCTGAGTCTTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAAGACTCAGAGATGTACTCGAGTACATCTCTGAGTCTTCATGG-3'

Other clones with same target sequence:

  1. TRCN0000071673