Construct: shRNA TRCN0000318006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019791.2-1889s21c1
DNA Barcode:
CCTCTTAGTGATTCTGGGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Maged1 (94275)
Gene Description:
melanoma antigen, family D, 1
Transcript:
RefSeq NM_019791.2 (CURRENT)
Match location:
Position 1889 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTAGTGATTCTGGGCAT
Hairpin Sequence:
5'-CCGG-CCTCTTAGTGATTCTGGGCAT-CTCGAG-ATGCCCAGAATCACTAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTAGTGATTCTGGGCATCTCGAGATGCCCAGAATCACTAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTAGTGATTCTGGGCATCTCGAGATGCCCAGAATCACTAAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000094273