Transcript: Mouse NM_019791.2

Mus musculus melanoma antigen, family D, 1 (Maged1), mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Maged1 (94275)
Length:
2752
CDS:
213..2540

Additional Resources:

NCBI RefSeq record:
NM_019791.2
NBCI Gene record:
Maged1 (94275)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019791.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000376093 ACTTACGACCCTCACCTAATC pLKO_005 1525 CDS 100% 10.800 15.120 N Maged1 n/a
2 TRCN0000094269 GCTCATCATTTGCAATAGTTT pLKO.1 2554 3UTR 100% 5.625 7.875 N Maged1 n/a
3 TRCN0000318007 GCTCATCATTTGCAATAGTTT pLKO_005 2554 3UTR 100% 5.625 7.875 N Maged1 n/a
4 TRCN0000314135 GTACCACCTGACTGGTCAATG pLKO_005 1419 CDS 100% 0.000 0.000 N Maged1 n/a
5 TRCN0000094273 CCTCTTAGTGATTCTGGGCAT pLKO.1 1889 CDS 100% 2.160 1.728 N Maged1 n/a
6 TRCN0000318006 CCTCTTAGTGATTCTGGGCAT pLKO_005 1889 CDS 100% 2.160 1.728 N Maged1 n/a
7 TRCN0000094270 CGCAAGGTTAATAACTTGAAT pLKO.1 945 CDS 100% 5.625 3.938 N Maged1 n/a
8 TRCN0000349533 CGCAAGGTTAATAACTTGAAT pLKO_005 945 CDS 100% 5.625 3.938 N Maged1 n/a
9 TRCN0000004238 CAAGATGAAAGTGCTGAGATT pLKO.1 2132 CDS 100% 4.950 3.465 N MAGED1 n/a
10 TRCN0000286024 CAAGATGAAAGTGCTGAGATT pLKO_005 2132 CDS 100% 4.950 3.465 N MAGED1 n/a
11 TRCN0000094272 CGAGAATACACTGATGTCTAT pLKO.1 1710 CDS 100% 4.950 3.465 N Maged1 n/a
12 TRCN0000094271 GCGGCCTTTAAGTCACAGAAT pLKO.1 546 CDS 100% 4.950 3.465 N Maged1 n/a
13 TRCN0000004235 CAGAATGTAAATCAGGCCAAA pLKO.1 801 CDS 100% 4.050 2.835 N MAGED1 n/a
14 TRCN0000286028 CAGAATGTAAATCAGGCCAAA pLKO_005 801 CDS 100% 4.050 2.835 N MAGED1 n/a
15 TRCN0000423583 AGCATCTGTATATTCTCATCG pLKO_005 1807 CDS 100% 4.050 2.835 N LAIR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019791.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02175 pDONR223 100% 86.8% 86.7% None (many diffs) n/a
2 ccsbBroad304_02175 pLX_304 0% 86.8% 86.7% V5 (many diffs) n/a
3 TRCN0000478258 TCCGGAGGTATTCTTATTGCTCAA pLX_317 15.6% 86.8% 86.7% V5 (many diffs) n/a
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