Construct: shRNA TRCN0000318039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010582.3-1235s21c1
DNA Barcode:
GCCAAGAGATACATTGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itih2 (16425)
Gene Description:
inter-alpha trypsin inhibitor, heavy chain 2
Transcript:
RefSeq NM_010582.3 (CURRENT)
Match location:
Position 1235 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAGATACATTGAGAAA
Hairpin Sequence:
5'-CCGG-GCCAAGAGATACATTGAGAAA-CTCGAG-TTTCTCAATGTATCTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAGATACATTGAGAAACTCGAGTTTCTCAATGTATCTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAGATACATTGAGAAACTCGAGTTTCTCAATGTATCTCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000080234