Construct: shRNA TRCN0000318043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145600.1-224s21c1
DNA Barcode:
CGGCAGAAGAATAGAGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp330 (30932)
Gene Description:
zinc finger protein 330
Transcript:
RefSeq NM_145600.1 (CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAGAAGAATAGAGCATTT
Hairpin Sequence:
5'-CCGG-CGGCAGAAGAATAGAGCATTT-CTCGAG-AAATGCTCTATTCTTCTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAGAAGAATAGAGCATTTCTCGAGAAATGCTCTATTCTTCTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAGAAGAATAGAGCATTTCTCGAGAAATGCTCTATTCTTCTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000098707