Construct: shRNA TRCN0000318101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016963.2-513s21c1
DNA Barcode:
CGCAGACTCTTACAGAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmod3 (50875)
Gene Description:
tropomodulin 3
Transcript:
RefSeq NM_016963.2 (CURRENT)
Match location:
Position 513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGACTCTTACAGAAGAAA
Hairpin Sequence:
5'-CCGG-CGCAGACTCTTACAGAAGAAA-CTCGAG-TTTCTTCTGTAAGAGTCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGACTCTTACAGAAGAAACTCGAGTTTCTTCTGTAAGAGTCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGACTCTTACAGAAGAAACTCGAGTTTCTTCTGTAAGAGTCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000089706