Transcript: Mouse NM_016963.2

Mus musculus tropomodulin 3 (Tmod3), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Tmod3 (50875)
Length:
3590
CDS:
200..1258

Additional Resources:

NCBI RefSeq record:
NM_016963.2
NBCI Gene record:
Tmod3 (50875)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_016963.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000089704 CCCGTCTTGTTGAAGTTAATT pLKO.1 792 CDS 100% 15.000 21.000 N Tmod3 n/a
2 TRCN0000089705 GCCCGTCTTGTTGAAGTTAAT pLKO.1 791 CDS 100% 13.200 18.480 N Tmod3 n/a
3 TRCN0000318102 GCCCGTCTTGTTGAAGTTAAT pLKO_005 791 CDS 100% 13.200 18.480 N Tmod3 n/a
4 TRCN0000089707 GCTCCTTTCTTACCTAGAGAA pLKO.1 403 CDS 100% 4.950 6.930 N Tmod3 n/a
5 TRCN0000318100 GCTCCTTTCTTACCTAGAGAA pLKO_005 403 CDS 100% 4.950 6.930 N Tmod3 n/a
6 TRCN0000091813 CGTTAGTTAGAATGAGGGTTT pLKO.1 2496 3UTR 100% 4.050 3.240 N LOC434449 n/a
7 TRCN0000089703 CCCACTGCATACCTTGTTAAA pLKO.1 1877 3UTR 100% 13.200 9.240 N Tmod3 n/a
8 TRCN0000318103 CCCACTGCATACCTTGTTAAA pLKO_005 1877 3UTR 100% 13.200 9.240 N Tmod3 n/a
9 TRCN0000089706 CGCAGACTCTTACAGAAGAAA pLKO.1 513 CDS 100% 5.625 3.938 N Tmod3 n/a
10 TRCN0000318101 CGCAGACTCTTACAGAAGAAA pLKO_005 513 CDS 100% 5.625 3.938 N Tmod3 n/a
11 TRCN0000091816 GCACCTCAGTAGAATTAGAAA pLKO.1 1092 CDS 100% 5.625 3.938 N LOC434449 n/a
12 TRCN0000091817 AGAATTAGAAATGGCCAAGAT pLKO.1 1102 CDS 100% 4.950 3.465 N LOC434449 n/a
13 TRCN0000091814 TGCAAAGACTTTGGAAGCCAA pLKO.1 856 CDS 100% 2.640 1.848 N LOC434449 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016963.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03090 pDONR223 100% 86.6% 90% None (many diffs) n/a
2 ccsbBroad304_03090 pLX_304 0% 86.6% 90% V5 (many diffs) n/a
3 TRCN0000470700 ACCTAGTCTGGCCTCACGTTAAGG pLX_317 32.9% 86.6% 90% V5 (many diffs) n/a
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