Construct: shRNA TRCN0000318134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177242.4-3103s21c1
DNA Barcode:
CCGACATTTAATTCCTGCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pptc7 (320717)
Gene Description:
PTC7 protein phosphatase homolog
Transcript:
RefSeq NM_177242.4 (CURRENT)
Match location:
Position 3103 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACATTTAATTCCTGCTAA
Hairpin Sequence:
5'-CCGG-CCGACATTTAATTCCTGCTAA-CTCGAG-TTAGCAGGAATTAAATGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACATTTAATTCCTGCTAACTCGAGTTAGCAGGAATTAAATGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACATTTAATTCCTGCTAACTCGAGTTAGCAGGAATTAAATGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000080893