Construct: shRNA TRCN0000318328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011249.2-980s21c1
DNA Barcode:
GCCGTGAACAAGGAATATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbl1 (19650)
Gene Description:
retinoblastoma-like 1 (p107)
Transcript:
RefSeq NM_011249.2 (CURRENT)
Match location:
Position 980 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGAACAAGGAATATGAA
Hairpin Sequence:
5'-CCGG-GCCGTGAACAAGGAATATGAA-CTCGAG-TTCATATTCCTTGTTCACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGAACAAGGAATATGAACTCGAGTTCATATTCCTTGTTCACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGAACAAGGAATATGAACTCGAGTTCATATTCCTTGTTCACGGC-3'

Other clones with same target sequence:

  1. TRCN0000088104