Transcript: Mouse NM_011249.2

Mus musculus retinoblastoma-like 1 (p107) (Rbl1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Rbl1 (19650)
Length:
4909
CDS:
80..3271

Additional Resources:

NCBI RefSeq record:
NM_011249.2
NBCI Gene record:
Rbl1 (19650)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_011249.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234086 CTTACTGGACGGCGGTATTTA pLKO_005 1190 CDS 100% 15.000 21.000 N Rbl1 n/a
2 TRCN0000257257 CCGTACTTTCCCGTGGATTAT pLKO_005 1627 CDS 100% 13.200 18.480 N Rbl1 n/a
3 TRCN0000040019 CCAAGCTAATAGTCACGTATA pLKO.1 2692 CDS 100% 10.800 15.120 N RBL1 n/a
4 TRCN0000088105 CGTGCATCATTGCTGTACTTT pLKO.1 816 CDS 100% 5.625 7.875 N Rbl1 n/a
5 TRCN0000088107 GCCCTGATTTAATGAAAGATA pLKO.1 2568 CDS 100% 5.625 7.875 N Rbl1 n/a
6 TRCN0000218550 ATCTTTGCCAATGCTATAATG pLKO_005 704 CDS 100% 13.200 10.560 N Rbl1 n/a
7 TRCN0000234088 ATATCCAGTGCTGGCTAATTT pLKO_005 4509 3UTR 100% 15.000 10.500 N Rbl1 n/a
8 TRCN0000375931 GAGTTTGGCTTGGACTAATAA pLKO_005 1771 CDS 100% 15.000 10.500 N Rbl1 n/a
9 TRCN0000234087 TATCCAATCAGGACCATATAA pLKO_005 2916 CDS 100% 15.000 10.500 N Rbl1 n/a
10 TRCN0000375930 ACACACTGATGGATAAGATTA pLKO_005 2082 CDS 100% 13.200 9.240 N Rbl1 n/a
11 TRCN0000376001 GACTTGTTAAATCCATCATTT pLKO_005 740 CDS 100% 13.200 9.240 N Rbl1 n/a
12 TRCN0000088103 GCTTGATGTTATCACCCTATA pLKO.1 3800 3UTR 100% 10.800 7.560 N Rbl1 n/a
13 TRCN0000376002 TGCAACCATTGTCTCCGATTT pLKO_005 1986 CDS 100% 10.800 7.560 N Rbl1 n/a
14 TRCN0000088104 GCCGTGAACAAGGAATATGAA pLKO.1 980 CDS 100% 5.625 3.938 N Rbl1 n/a
15 TRCN0000318328 GCCGTGAACAAGGAATATGAA pLKO_005 980 CDS 100% 5.625 3.938 N Rbl1 n/a
16 TRCN0000088106 CCGTGCATCATTGCTGTACTT pLKO.1 815 CDS 100% 4.950 3.465 N Rbl1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_011249.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13940 pDONR223 100% 82.7% .7% None (many diffs) n/a
2 ccsbBroad304_13940 pLX_304 0% 82.7% .7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000478961 GAAAGCGCCTCCCTATTTAAGAGC pLX_317 13.2% 82.7% .7% V5 (not translated due to prior stop codon) (many diffs) n/a
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