Construct: shRNA TRCN0000318773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058243.2-1707s21c1
DNA Barcode:
CAGTGACAGTTCGACTGATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD4 (23476)
Gene Description:
bromodomain containing 4
Transcript:
RefSeq NM_058243.2 (CURRENT)
Match location:
Position 1707 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGACAGTTCGACTGATGA
Hairpin Sequence:
5'-CCGG-CAGTGACAGTTCGACTGATGA-CTCGAG-TCATCAGTCGAACTGTCACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGACAGTTCGACTGATGACTCGAGTCATCAGTCGAACTGTCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGACAGTTCGACTGATGACTCGAGTCATCAGTCGAACTGTCACTG-3'

Other clones with same target sequence:

  1. TRCN0000199427