Construct: shRNA TRCN0000319306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032704.3-1376s21c1
DNA Barcode:
GCTGCCCTTGAGAAGGATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBA1C (84790)
Gene Description:
tubulin alpha 1c
Transcript:
RefSeq NM_032704.3 (NON-CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCCTTGAGAAGGATTAT
Hairpin Sequence:
5'-CCGG-GCTGCCCTTGAGAAGGATTAT-CTCGAG-ATAATCCTTCTCAAGGGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCCTTGAGAAGGATTATCTCGAGATAATCCTTCTCAAGGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCCTTGAGAAGGATTATCTCGAGATAATCCTTCTCAAGGGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000157025
  2. TRCN0000029141