Construct: shRNA TRCN0000157025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006082.1-1343s1c1
DNA Barcode:
GCTGCCCTTGAGAAGGATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBA1B (10376)
Gene Description:
tubulin alpha 1b
Transcript:
RefSeq NM_006082.1 (NON-CURRENT)
Match location:
Position 1343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCCTTGAGAAGGATTAT
Hairpin Sequence:
5'-CCGG-GCTGCCCTTGAGAAGGATTAT-CTCGAG-ATAATCCTTCTCAAGGGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCCTTGAGAAGGATTATCTCGAGATAATCCTTCTCAAGGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCCTTGAGAAGGATTATCTCGAGATAATCCTTCTCAAGGGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000029141
  2. TRCN0000319306