Construct: shRNA TRCN0000319587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016917.2-905s21c1
DNA Barcode:
GTCGGCCAGATTATGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc40a1 (53945)
Gene Description:
solute carrier family 40 (iron-regulated transporter), member 1
Transcript:
RefSeq NM_016917.2 (CURRENT)
Match location:
Position 905 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGGCCAGATTATGACATTT
Hairpin Sequence:
5'-CCGG-GTCGGCCAGATTATGACATTT-CTCGAG-AAATGTCATAATCTGGCCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGGCCAGATTATGACATTTCTCGAGAAATGTCATAATCTGGCCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGGCCAGATTATGACATTTCTCGAGAAATGTCATAATCTGGCCGAC-3'

Other clones with same target sequence:

(none)